Which of the following tools of recombinant DNA technology are incorrectly paired with its use?

1. Tardigrade
2. Question
3. Biology
4. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

 1974  42 Biotechnology : Principles and Processes Report Error

A

EcoRI - Production of sticky ends

B

ligase - Multiplication of molecules

C

ori- copy number

D

Selectable marker - Identification of transformants

Solution:

ligases are also called genetic gum. They join two individual fragments of double stranded by forming phosphodiester bonds between them thus help in sealing of fragments. Therefore acts as molecular glue. The enzyme used most often is ligase.

EcoR I - production of sticky ends

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DNA ligase - multiplication of rDNA molecules

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DNA Polymerase - polymerase chain reaction (PCR) to amplify the DNA segments

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Selectable marker - identification of transformants

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Solution

The correct option is B DNA ligase - multiplication of rDNA moleculesDNA ligase is an enzyme which joins or seals the gaps between DNA fragments by formation of phosphodiester bonds. It has no role to play in the multiplication of rDNA molecules. EcoR I is a restriction endonuclease that recognises a specific palindromic sequence and makes cuts at these specific locations to form sticky ends in the DNA. The ends with single-stranded DNA “overhangs” are called sticky ends. DNA polymerase enzyme helps in the synthesis of a DNA strand by adding deoxyribonucleotides to the 3' end of a primer. It is used in polymerase chain reactions (PCR) for amplifying the DNA segments. Selectable markers are genes that help in identification and selection of transformants over non-transformants. Transformants are those cells that have undergone genetic transformation through the uptake of foreign DNA. Non-transformants are those cells which have not taken up the foreign DNA. Generally, genes that confer resistance to organisms against antibiotics, serve as selectable markers.

EcoRl -Production of sticky ends DNA ligase - Multiplication of DNA molecules ori- copy number Selectable market - Identification of transformants

Answer : B

Solution : `DNA` ligases are also called genetic gum. They join two indiviual fragments of double stranded DNA by forming phosphodiester bonds between them thus help in sealing of DNA fragments. Therefore acts as molecular glue. The enzyme used most often is `T_(4)` DNA ligase.

Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?

A.	restriction endonuclease - production of DNA fragments for gene cloning.
B.	DNA ligase - enzyme that cuts DNA, creating sticky ends.
C.	DNA polymerase - copies DNA sequences in the polymerase chain reaction.
D.	reverse transcriptase - production of cDNA from mRNA.
E.	electrophoresis - RLFP analysis.

Which tools of recombinant DNA technology is incorrectly paired with its use?

Which of the following tool is not used in recombinant DNA technology?

Which of the following are the tools used in recombinant DNA technology?

Which of the following is not a tool in recombinant DNA technology MHT CET 2020?